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htlr8  (InvivoGen)


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Bioz Manufacturer Symbol InvivoGen manufactures this product  
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    Structured Review

    InvivoGen htlr8
    Htlr8, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/htlr8+cells/HEK-Blue+hTLR9+cells/pm41962328-330-16-21
    Average 95 stars, based on 130 article reviews
    htlr8 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Control:

    Article Title: Neurodegenerative disease-associated microRNAs acting as signaling molecules modulate CNS neuron structure and viability
    Article Snippet: .. HEK-BlueTM mTLR7, mTLR8, hTLR7, and hTLR8 cells and respective control Null2-k, Null1-v, Null1-k, and Null1 cells (Invivogen, San Diego, CA, USA) were cultured according to the manufacturer’s instructions. .. The growth medium consisted of Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen #41965062, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco #10082–147, Thermo Fisher Scientific, Waltham, MA, USA), 100 U/mL penicillin/100 μg/mL streptomycin (1% P/S, Gibco #15140–122, Thermo Fisher Scientific, Waltham, MA, USA), 100 μg/ml zeocin (Invivogen #ant-zn-05, San Diego, CA, USA), and 10–30 μg/ml blasticidin (Invivogen #ant-bl-05, San Diego, CA, USA).

    Article Title: Neurodegenerative disease-associated microRNAs acting as signaling molecules modulate CNS neuron structure and viability.
    Article Snippet: .. HEK-BlueTM mTLR7, mTLR8, hTLR7, and hTLR8 cells and respective control Null2-k, Null1-v, Null1-k, and Null1 cells (Invivogen, San Diego, CA, USA) were cultured according to the manufacturer’s instructions. .. The growth medium consisted of Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen #41965062, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco #10082–147, Thermo Fisher Scientific, Waltham, MA, USA), 100 U/mL penicillin/100 μg/mL streptomycin (1% P/S, Gibco #15140–122, Thermo Fisher Scientific, Waltham, MA, USA), 100 μg/ml zeocin (Invivogen #ant-zn-05, San Diego, CA, USA), and 10–30 μg/ml blasticidin (Invivogen #ant-bl-05, San Diego, CA, USA).

    Cell Culture:

    Article Title: Neurodegenerative disease-associated microRNAs acting as signaling molecules modulate CNS neuron structure and viability
    Article Snippet: .. HEK-BlueTM mTLR7, mTLR8, hTLR7, and hTLR8 cells and respective control Null2-k, Null1-v, Null1-k, and Null1 cells (Invivogen, San Diego, CA, USA) were cultured according to the manufacturer’s instructions. .. The growth medium consisted of Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen #41965062, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco #10082–147, Thermo Fisher Scientific, Waltham, MA, USA), 100 U/mL penicillin/100 μg/mL streptomycin (1% P/S, Gibco #15140–122, Thermo Fisher Scientific, Waltham, MA, USA), 100 μg/ml zeocin (Invivogen #ant-zn-05, San Diego, CA, USA), and 10–30 μg/ml blasticidin (Invivogen #ant-bl-05, San Diego, CA, USA).

    Article Title: Strategic Development of a Self-Adjuvanting Sars-Cov-2 Rbd Vaccine: From Adjuvant Screening to Enhanced Immunogenicity with a Modified Tlr7 Agonist
    Article Snippet: Analysis of the final RBD-MT conjugate by MALDI-TOF mass spectrometry indicated that molecule covalently links with the RBD. .. 2.8 TLR reporter assay HEK-Blue hTLR7 and hTLR8 cells (InvivoGen) were cultured in DMEM supplemented with 10% FBS, Penicillin-Streptomycin (Beyotime), blasticidin (InvivoGen), zeocin (InvivoGen) and normocin (InvivoGen). .. HEK-Blue hTLR7 cells were harvested and mixed with HEK-BlueTM Detection (InvivoGen), the mixture was distributed into 96 well plates at 2.5×104 cells/well then treated with DMSO, TLR7/8 or compound 6 at different concentrations (0.0001 μM, 0.001 μM, 0.01 μM, 0.1 μM, 1 μM) for 14 h. HEK-Blue hTLR8 cells were harvested and mixed with HEKBlueTM Detection (InvivoGen), the mixture was distributed into 96 well plates at 2.5×104 cells/well then treated with DMSO, TLR7/8 or compound 6 at different concentrations (0.015 μM, 0.061 μM, 0.24 μM, 0.98 μM, 3.9 μM, 15 μM, 62.5 μM) for 14 h. Determine SEAP activity by reading the optical density (OD) at 630 nm on SpectraMax-M2 (Molecular Devices) plate reader.

    Article Title: The novel selective TLR7 agonist GY101 suppresses colon cancer growth by stimulating immune cells.
    Article Snippet: Toll-like receptor (TLR) 7, a transmembrane signal transduction receptor expressed on the surface of endosomes, has become an attractive target for antiviral and cancer immunotherapies.. TLR7 can induce signal transduction by recognizing single-stranded RNA or its analogs, leading to the release of cytokines such as IL-6, IL-12, TNF-α and type-I IFN.. Activation of TLR7 helps to enhance immunogenicity and immune memory by stimulating immune cells.

    Article Title: Neurodegenerative disease-associated microRNAs acting as signaling molecules modulate CNS neuron structure and viability.
    Article Snippet: .. HEK-BlueTM mTLR7, mTLR8, hTLR7, and hTLR8 cells and respective control Null2-k, Null1-v, Null1-k, and Null1 cells (Invivogen, San Diego, CA, USA) were cultured according to the manufacturer’s instructions. .. The growth medium consisted of Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen #41965062, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco #10082–147, Thermo Fisher Scientific, Waltham, MA, USA), 100 U/mL penicillin/100 μg/mL streptomycin (1% P/S, Gibco #15140–122, Thermo Fisher Scientific, Waltham, MA, USA), 100 μg/ml zeocin (Invivogen #ant-zn-05, San Diego, CA, USA), and 10–30 μg/ml blasticidin (Invivogen #ant-bl-05, San Diego, CA, USA).

    Article Title: Strategic development of a self-adjuvanting SARS-CoV-2 RBD vaccine: From adjuvant screening to enhanced immunogenicity with a modified TLR7 agonist.
    Article Snippet: Adjuvants enhance the body’s immune response to a vaccine, often leading to better protection against diseases.. Monophosphoryl lipid A analogues (MPLA, TLR4 agonists), α-galactosylceramide analogues (NKT cell agonists), and imidazoquinoline compounds (TLR7/8 agonists) are emerging novel adjuvants on market or under clinical trials.. Despite significant interest in these adjuvants, a direct comparison of their adjuvant activities remains unexplored.

    Activity Assay:

    Article Title: Strategic Development of a Self-Adjuvanting Sars-Cov-2 Rbd Vaccine: From Adjuvant Screening to Enhanced Immunogenicity with a Modified Tlr7 Agonist
    Article Snippet: 2.8 TLR reporter assay HEK-Blue hTLR7 and hTLR8 cells (InvivoGen) were cultured in DMEM supplemented with 10% FBS, Penicillin-Streptomycin (Beyotime), blasticidin (InvivoGen), zeocin (InvivoGen) and normocin (InvivoGen). .. HEK-Blue hTLR7 cells were harvested and mixed with HEK-BlueTM Detection (InvivoGen), the mixture was distributed into 96 well plates at 2.5×104 cells/well then treated with DMSO, TLR7/8 or compound 6 at different concentrations (0.0001 μM, 0.001 μM, 0.01 μM, 0.1 μM, 1 μM) for 14 h. HEK-Blue hTLR8 cells were harvested and mixed with HEKBlueTM Detection (InvivoGen), the mixture was distributed into 96 well plates at 2.5×104 cells/well then treated with DMSO, TLR7/8 or compound 6 at different concentrations (0.015 μM, 0.061 μM, 0.24 μM, 0.98 μM, 3.9 μM, 15 μM, 62.5 μM) for 14 h. Determine SEAP activity by reading the optical density (OD) at 630 nm on SpectraMax-M2 (Molecular Devices) plate reader. ..

    Article Title: Strategic development of a self-adjuvanting SARS-CoV-2 RBD vaccine: From adjuvant screening to enhanced immunogenicity with a modified TLR7 agonist.
    Article Snippet: Adjuvants enhance the body’s immune response to a vaccine, often leading to better protection against diseases.. Monophosphoryl lipid A analogues (MPLA, TLR4 agonists), α-galactosylceramide analogues (NKT cell agonists), and imidazoquinoline compounds (TLR7/8 agonists) are emerging novel adjuvants on market or under clinical trials.. Despite significant interest in these adjuvants, a direct comparison of their adjuvant activities remains unexplored.

    Reporter Assay:

    Article Title: Strategic Development of a Self-Adjuvanting Sars-Cov-2 Rbd Vaccine: From Adjuvant Screening to Enhanced Immunogenicity with a Modified Tlr7 Agonist
    Article Snippet: Analysis of the final RBD-MT conjugate by MALDI-TOF mass spectrometry indicated that molecule covalently links with the RBD. .. 2.8 TLR reporter assay HEK-Blue hTLR7 and hTLR8 cells (InvivoGen) were cultured in DMEM supplemented with 10% FBS, Penicillin-Streptomycin (Beyotime), blasticidin (InvivoGen), zeocin (InvivoGen) and normocin (InvivoGen). .. HEK-Blue hTLR7 cells were harvested and mixed with HEK-BlueTM Detection (InvivoGen), the mixture was distributed into 96 well plates at 2.5×104 cells/well then treated with DMSO, TLR7/8 or compound 6 at different concentrations (0.0001 μM, 0.001 μM, 0.01 μM, 0.1 μM, 1 μM) for 14 h. HEK-Blue hTLR8 cells were harvested and mixed with HEKBlueTM Detection (InvivoGen), the mixture was distributed into 96 well plates at 2.5×104 cells/well then treated with DMSO, TLR7/8 or compound 6 at different concentrations (0.015 μM, 0.061 μM, 0.24 μM, 0.98 μM, 3.9 μM, 15 μM, 62.5 μM) for 14 h. Determine SEAP activity by reading the optical density (OD) at 630 nm on SpectraMax-M2 (Molecular Devices) plate reader.



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